Aggregated proteoglycan synthesis in organ cultures of human nucleus pulposus.
نویسندگان
چکیده
منابع مشابه
Notochordal cell rich nucleus pulposus tissue increases proteoglycan accumulation and promotes a healthy nucleus pulposus phenotype in human MSCs
pulposus phenotype in human MSCs +Purmessur, D; Godburn K; Abbott, RD: Schek RM; Iatridis JC +University of Vermont, Burlington VT Senior author [email protected] INTRODUCTION Mesenchymal stem cells (MSCs) are an attractive cell source for IVD repair, yet the means of differentiating these cells to a nucleus pulposus (NP) phenotype is not clear and an area of high priority. Notochordal cel...
متن کاملSenescence of Nucleus Pulposus Chondrocytes in Human Intervertebral Discs
STUDY DESIGN Senescence-related markers were assessed in surgically obtained human nucleus pulposus (NP) specimens. PURPOSE To demonstrate the mechanism and signaling pathway involved in the senescence of NP chondrocytes. OVERVIEW OF LITERATURE The population of senescent disc cells has been shown to be increased in degenerated or herniated discs. However, the mechanism and signaling pathwa...
متن کاملNotochordal conditioned media from tissue increases proteoglycan accumulation and promotes a healthy nucleus pulposus phenotype in human mesenchymal stem cells
INTRODUCTION Notochordal cells (NCs) are influential in development of the intervertebral disc (IVD) and species that retain NCs do not degenerate. IVD repair using bone marrow derived mesenchymal stem cells (MSCs) is an attractive approach and the harsh microenvironment of the IVD suggests pre-differentiation is a necessary first step. The goal of this study was to use soluble factors from NCs...
متن کاملCartilage tissue enhances proteoglycan retention by nucleus pulposus cells in vitro.
OBJECTIVE To investigate the effect of cartilage on nucleus pulposus (NP) tissue in an in vitro model. METHODS Cells were isolated from bovine NP or articular cartilage and allowed to form tissue in vitro. The NP tissue was grown either alone or in the presence of cartilage tissue (coculture) for up to 4 weeks and examined for histologic appearance, gene expression, and biochemical compositio...
متن کاملLow-intensity pulsed ultrasound stimulates cell proliferation, proteoglycan synthesis and expression of growth factor-related genes in human nucleus pulposus cell line.
Low-intensity pulsed ultrasound (LIPUS) stimulation has been shown to effect differentiation and activation of human chondrocytes. A study involving stimulation of rabbit disc cells with LIPUS revealed upregulation of cell proliferation and proteoglycan (PG) synthesis. However, the effect of LIPUS on human nucleus pulposus cells has not been investigated. In the present study, therefore, we inv...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1979
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)86555-9